Immunoassay of Collagen Crosslinks in Acidified Urine
نویسندگان
چکیده
منابع مشابه
Quantitative determination of collagen crosslinks.
The primary functional role of collagen is as a supporting tissue and it is now established that the aggregated forms of the collagen monomers are stabilised to provide mechanical strength by a series of intermolecular cross-links. In order to understand the mechanical properties of collagen, it is necessary to identify and quantitatively determine the concentration of the cross-links during th...
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We describe an enzyme immunoassay for determination of total estriol in urine. Estriol covalently bound to horseradish peroxidase is used as tracer, and free and bound hormone are separated by precipitation with polyethylene glycol. The method can be used with either acid hydrolysis at 100 degrees C for 30 min or enzyme hydrolysis at 50 degrees C for 40 min; results by the former procedure are ...
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OBJECTIVE To investigate the relationship between inflammation markers and content of pyridinium crosslinks in hydrolysates of synovial tissue and to specify the significance of urinary excreted pyridinoline, released primarily from collagen I and II of bone and cartilage, and deoxypyridinoline released especially from collagen I of bone and dentin, dependent on disease activity in rheumatoid a...
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Reduction of insoluble collagens from bone, skin, and tendon with NsB3H4 produced several radioactive substances which were isolated from acid hydrolysates of the protein. One of these substances, aldol-histidine, was most abundant in cow skin collagen and also appeared in reconstituted fibrils of purified cow skin tropocollagen. Another compound, histidino-hydroxymerodesmosine, was abundant in...
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ژورنال
عنوان ژورنال: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
سال: 1995
ISSN: 0004-5632,1758-1001
DOI: 10.1177/000456329503200311